Autologous Transplantation of the Retinal Pigment Epithelium and Choroid in the Treatment of Age-Related Macular Degeneration

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Autologous Transplantation of the Retinal Pigment Epithelium and Choroid in the Treatment of Age-Related Macular Degeneration


Antonio López Bolaños and Rosa Evangelina Garnica Hayashi


 


In the daily practice of ophthalmology, we encounter diseases whose treatments escape our hands. The physiopathology of these diseases includes damage or atrophy of retinal pigment epithelium (RPE), retinitis pigmentosa, geographic atrophy in age-related macular degeneration (AMD), RPE tears, and disciform scars.


RETINAL PIGMENT EPITHELIUM


There are approximately 3.5 million RPE cells in the adult human eye (1). The RPE cells are located between the neural retina and the vascular choroid and play a critical role in the maintenance of visual function. The functions of the RPE include phagocytosis of shed photoreceptor outer segments, metabolism of retinol, formation of the outer blood-retinal barrier, maintenance of the extracellular matrix, and regulation of ion and metabolite transport.


The RPE cells engulf and degrade shed rod outer segments at a very high rate: one human RPE cell will ingest and degrade about 3000 million discs during a 70 year lifespan. The apical surface of the RPE cells interface with the outer segments of the photoreceptors, while the RPE basal domain is attached firmly to the underlying Bruch’s membrane. The basement membrane of the RPE represents the innermost layer of Bruch’s membrane. RPE cells exhibit regional differences in morphology and function. In the macula, RPE cells are smaller and columnar in appearance, while in the periphery, they are larger and cuboidal.


In RPE cultures, the enzymatic capacity of RPE cells has been shown to be age independent (2), contrary to what may occur in vivo, where with age, degradation of outer segments of the photoreceptors appears to be diminished. With age and pathologic changes in the human eye, degradation of outer segment materials within the phagolysosomes may lead to the formation of lipofuscin granules composed of outer segments of the photoreceptors residue (3, 4). In response to injury or certain alterations in the microenvironment, RPE cells, which in situ do not proliferate, may detach from their substratum, migrate, proliferate, and acquire a macrophage-like or fibroblast-like morphology (5, 6). These morphologic and functional changes, associated with alterations on gene expression, may be referred to as activation. Activation events include physical trauma with displacement to a new environment, accumulation of intraocular blood, breakdown of the blood-retinal barrier with inflammatory cell infiltration, alteration of components of the extracellular matrix, alterations in choroidal circulation, and diffusion of oxygen to the RPE layer.


RPE shows specific features associated with aging. The proportion of apoptotic human RPE cells increases significantly with age, and these apoptotic cells are confined mainly to the macula (7). Peripheral RPE cells may compensate for the death of macular RPE cells. Furthermore, cells become more irregular in size and shape, deposits of RPE-derived material accumulate in Bruch’s membrane and lipofuscin appears in the cells’ cytoplasm (813).


ADVANCES IN TREATMENT OF AGE-RELATED MACULAR DEGENERATION


Nowadays, the possibility of rebuilding a damaged retina has been subject of widespread research. The field of AMD treatment has witnessed a number of significant advances.


AMD is a progressive disease of the macula that in its late or advanced stage results in loss of central vision. Visual loss in late AMD is a consequence of one of two processes that cause photoreceptor dysfunction- geographic atrophy or choroidal neovascularization (CNV). In geographic atrophy, there is confluent atrophy of the choriocapillaries and associated RPE. In CNV, an ingrowth of new vessels occurs from the choriocapillaries that breaches Bruch’s membrane and the RPE to invade the retina. These fenestrated and friable new vessels leak serous fluid, lipids, and blood below and into the neural retina, with subsequent fibrous scarring, which defines the late stage of AMD. This scarring is called disciform scar and is generally associated with the loss of neural tissue from the retina. The dysfunction as well as death of photoreceptors is a major contributor to vision loss in AMD. Other types of retinal neuronal cells affected secondarily because of the loss of photoreceptors also lead to visual loss.


The disciform scars are classified anatomically as subneurosensory retinal, sub-RPE, or combined. Subneurosensory retinal lesions are defined by the absence of the RPE interposed between the lesion and the remaining retinal tissue.


There are no preventive treatments, however, there is some evidence that antioxidant vitamin and mineral supplementation can slow down the progression of the disease in people with moderate AMD.


In the beginning of the 1980’s, the Macular Photocoagulation Study Group reported favorable outcomes for thermal laser photocoagulation in a small proportion of eyes with well-delineated (or classic) small extrafoveal and juxtafoveal CNV lesions, but less favorable outcomes in patients with subfoveal CNV lesions due to the likelihood of immediate and severe vision loss from the laser treatment (1416). In the 1990’s, introduction of photodynamic therapy with verteporfin expanded the therapeutic options available to patients with subfoveal CNV, offering them the possibility of exudative AMD treatment. However, the treatment is not accompanied with improvement in visual acuity and can also cause some degree of retinal damage (17).


The role of surgery in the treatment of CNV-complicated AMD remains controversial. Although submacular removal of CNV was not favored in the randomized submacular surgery trials, patients who underwent hemorrhagic CNV showed significantly reduced risk of severe visual acuity loss and contrast sensitivity loss compared to the control patients after 24 months of follow-up (18, 19). Subfoveal membranectomy almost always results in the removal of the native RPE along with the choroidal neovascular complex in AMD eyes and leads to the development or progression of atrophy of the subfoveal choriocapillaris.


Other surgical treatments for AMD include pneumatic displacement of submacular blood with or without intravitreal or subretinal injection of recombinant tissue plasminogen activator and translocation of the macula to an extramacular and healthier RPE area (Machemer 1993). Macular translocation seems to stabilize and sometimes even improve visual acuity (20, 21). However, full macular translocation bears a considerable risk of proliferative vitreoretinopathy (PVR), diplopia, and macular edema.


Given the recent number of anti-VEGF therapies that have demonstrated efficacy in studies, the repertoire of treatments for neovascular AMD has significantly expanded. Bevacizumab, a full-length recombinant humanized monoclonal antibody against VEGF has been effective in AMD treatment (2224). Pegaptanib and ranibizumab have also been shown to be effective in large clinical trials. However, there are some patients who do not respond favorably to such anti-VEGF treatment (25, 26).


For patients with geographic atrophy in AMD, RPE tears, or disciform scars, the treatment available is still under discussion. Currently, studies are investigating the effect of RPE transplantation.


Transplantation of RPE in AMD was first performed by Algvere et al. (27, 28) by using heterologous fetal RPE. Unfortunately, all eyes developed macular edema, most likely as a result of host-graft rejection. Visual acuity declined and foveal fixation was lost in all patients. Iris pigment epithelium (IPE) was easily accessible; however, its functionality has not been established. Although the short-term results have been promising, there have been no long-term benefits. This led to the assumption that the transplantation of an intact monolayer is required to achieve a functional transplant.


Peyman et al. (29) first suggested translocation of peripheral choroid and RPE. Others later demonstrated the clinical feasibility of the new technique (3032).


RETINAL PIGMENT EPITHELIUM TRANSPLANTATION


Regenerating RPE cells by stem-cell therapy is a promising avenue for rebuild the damaged retina. Stem-cells are defined as pluripotent cells capable of differentiating into a variety of cell types and, under appropriate conditions, into a variety of tissues, including muscle, kidney, brain, blood, liver, skin, and retina. Stem cells can be divided into those that are isolated from fetal material (embryonic stem cells) and those from adult tissue (adult stem cells). The former are typically isolated from blastocysts and have pluripotency; that is, that they can give rise to virtually any adult tissue cell type under appropriate conditions. Adult-derived stem cells typically reside in adult tissues in a quiescent, undifferentiated state and under appropriate stimuli will divide and differentiate into the cell type of the tissue in which they reside or if appropriately stimulated into other cell types., It is however, not entirely clear whether this is a result of a stem cell differentiating into the end cell product or if this occurs by somatic cell fusion between the “stem” cell and a population of differentiated, postmitotic, tissue-specific cells that then share the stem cell’s proliferative capabilities as well as their own tissue-specific phenotypic characteristics (3334).


Damaged RPE cells and associated atrophy are hallmarks of AMD and heroic surgical approaches (3537) have been considered to provide photoreceptors in such individuals with healthier RPE-rich regions of the retina through retinal translocation and the insertion of RPE sheets. As RPE cells do not require synaptic reconnection, RPE transplantation may be less complex than that of photoreceptors and retinal neurons. To date, researchers have translocated RPE tissue into the fovea of AMD patients after choroidal neovascular membrane removal (38). Patients maintained foveal fixation early, but central visual function was found to be transient, with a decline in 5 to 6 years (39). Another group transplanted autologous RPE cells harvested from the nasal retina to the macular region in patients with AMD. This technique led to clinical benefit in some patients, but there was difficulty in collecting a sufficient number of autologous RPE cells (40). Autologous RPE transplants also have the disadvantage of carrying the same genetic predisposition that led to the original disease state.


Allograft RPE transplanted with the goal of treating retinal degeneration and preserving retinal function (41).


METHOD OF TRANSPLANTATION OF AUTOLOGOUS RETINAL PIGMENT EPITHELIUM CHOROID


Peyman et al. first reported the translocation of a free full-thickness autologous graft consisting of RPE, Bruch’s membrane, choriocapillaris and choroid taken from the paramacular region in one patient (29). This approach was refined by others (38, 42). Van Meurs pioneered a technique to cut out the graft from the midperiphery (42).


Patients with either subfoveal lesions or geographic atrophy that was a result of AMD, are candidates for the autologous RPE transplants accomplishment. The central reason for joint transplantation of the RPE and choroid is that, besides the epithelial damage, such patients also show ailment of the Bruch’s membrane and the choriocapillaris.


We recommend phacoemulsification in all patients before vitrectomy. (Fig. 56-1A) After the induction of a posterior vitreous detachment and a complete vitrectomy, it is very important to perform a good shaving of the vitreous base.


If necessary, depending on the case, the disciform scar can be removed from the subretinal space through a paramacular retinotomy in the temporal raphe in direction of the nervous fibers (Fig. 56-1B–C). Hydro dissection can be used for macular detachment and for preparing the place where the graft will be placed.


After a rectangle is marked with laser photocoagulation in the midperiphery, always at the superiors sectors and removal of the retina within the diathermia marks, vitreous scissors were used to cut an autologous peripheral full-thickness patch of RPE, Bruch membrane, and choroid. (Fig. 56-1D–F). The size of the graft is determined by the size of the macular atrophy.


The graft of the autologous RPE choroid is taken to the subretinal space and repositioned under the macula through the existing paramacular retinotomy (Fig. 56-1G). Perfluorocarbon liquid (PFCL) is injected to keep the graft in place (Fig. 56-1H). Surgery is completed with gas C3F8 or silicone-oil tamponade.




Figure 56-1. Surgical technique of transplantation of autologous retinal pigmentary epithelium choroid.

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Oct 21, 2016 | Posted by in OPHTHALMOLOGY | Comments Off on Autologous Transplantation of the Retinal Pigment Epithelium and Choroid in the Treatment of Age-Related Macular Degeneration

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