Granulomatous Inflammation

Introduction

Chronic granulomatous inflammation is a proliferative inflammation characterized by a cellular infiltrate of epithelioid cells [and sometimes inflammatory giant cells, lymphocytes, plasma cells, polymorphonuclear leukocytes (PMNs), and eosinophils; see Chapter 1].

Post-Traumatic

Sympathetic Uveitis [Sympathetic Ophthalmia (SO), Sympathetic Ophthalmitis]

I. SO (Figs 4.1 and 4.2) is a bilateral, diffuse, granulomatous, T-cell-mediated uveitis that occurs from two weeks to many years after penetrating or perforating ocular injury and is associated with traumatic uveal incarceration or prolapse.

A. Although the uveitis may start as early as five days or as late as 50 years after injury, well over 90% of cases occur after two weeks but within one year. Most of these (80%) occur within three weeks to three months post-injury.

B. Removal of the injured eye before sympathetic uveitis occurs usually completely protects against inflammation developing in the noninjured eye.¹ Once the inflammation starts, however, removal of the injured (“exciting”) eye probably has little effect on the course of the disease, especially after 3–6 months.

SO has been reported in nontraumatized eyes in a few isolated cases. However, unless the whole eye is serially sectioned and carefully examined for evidence of perforation, the clinician can never be sure that some long-forgotten penetrating ocular wound is not present. A diagnosis of sympathetic uveitis in the absence of an ocular injury should be viewed with marked skepticism.

C. SO affects approximately 0.03 per 100,000 persons per year and accounts for approximately 1 to 2% of uveitic patients. Greater than 50% of patients maintain a functional visual acuity better than 20/50. The condition seems to be decreasing in occurrences in recent years.

Fig. 4.1 Sympathetic uveitis. A, Large, “greasy,” mutton-fat keratic precipitates (KPs: collections of epithelioid cells, lymphocytes, and plasma cells on posterior surface of cornea) seen in patient with sympathetic uveitis. B, Another patient had a perforating injury to his eye. The other (sympathizing) eye developed photophobia and mutton-fat KPs; the injured (exciting) eye was enucleated. Radiograph of enucleated eye shows two metallic foreign bodies in eye. C, Gross specimen shows massive, diffuse thickening of choroid. D, Granulomatous inflammation fills and thickens the choroid (see also Fig. 4.2A and B). The Perl stain is positive (blue) for iron in the large, dark foreign body in the choroid. (B–D, Courtesy of Dr. TH Chou.)

Fig. 4.2 Sympathetic uveitis. A, Enucleated eye from Fig. 4.1B and C shows diffuse thickening of the choroid (shown with increased magnification in B) by granulomatous inflammation. The pale areas represent epithelioid cells, and the dark areas consist mainly of lymphocytes. C, Sparing of the choriocapillaris and pigment phagocytosis by epithelioid cells is seen. Note granulomatous inflammation of scleral canal in lower right corner (reason why evisceration does not protect against sympathetic uveitis). D, Dalen–Fuchs nodule of epithelioid cells between retinal pigment epithelium and Bruch’s membrane is seen. Underlying choriocapillaris is spared, and overlying neural retina is free of inflammatory process. (Courtesy of Dr. TH Chou.)

II. Blurred vision and photophobia in the noninjured (sympathizing) eye are usually the first symptoms. Vision and photophobia worsen concurrently in the injured (exciting) eye, and a granulomatous uveitis develops (see Fig. 4.1A).

Glaucoma may develop due to blockage of the angle by cellular debris or peripheral anterior synechiae. Hypotony may occur from decreased aqueous output by the inflamed ciliary body.

III. The cause appears to be a delayed-type hypersensitivity reaction of the uvea to antigens localized on the retinal pigment epithelium or on uveal melanocytes.

A. The lymphocytic infiltrate consists almost exclusively of T lymphocytes.

B. B cells found in some cases, usually of long duration, may represent the end stage of the disease.

Phacoanaphylactic endophthalmitis (PE) was found in approximately 25% of patients who had sympathetic uveitis in cases submitted to the Armed Forces Institute of Pathology before 1950, whereas since 1950, only approximately 5% of cases of sympathetic uveitis have also had PE. The marked reduction in the association of the two diseases is probably attributable to advances in the management of penetrating wounds. It has been suggested that sympathetic uveitis represents a forme fruste of Vogt–Koyanagi–Harada (VKH) syndrome [see subsection titled Vogt–Koyanagi–Harada Syndrome (Uveomeningoencaphalitic Syndrome)]. The relationship between the two entities is still uncertain.

C. Human leukocyte antigen (HLA)-DRB1*04, DQA1*03, and DQB1*04 are significantly associated with sympathetic uveitis.

IV. Histologically, SO has certain characteristics that are suggestive of the disorder but not diagnostic.

A. Sympathetic uveitis is a clinicopathologic diagnosis, never a histologic diagnosis alone.

The uveal inflammatory reaction tends to be more vigorous in black than in white patients.

B. The following four histologic findings are characteristic of both sympathizing and exciting eyes:

1. Diffuse granulomatous uveal inflammation composed predominantly of epithelioid cells and lymphocytes. Eosinophils may be plentiful. Plasma cells are few or moderate in number. Neutrophils are rare or absent.

2. Sparing of the choriocapillaris.

3. Epithelioid cells containing phagocytosed uveal pigment.

4. Dalen–Fuchs nodules (i.e., collections of epithelioid cells lying between Bruch’s membrane and the retinal pigment epithelium with no involvement of the overlying neural retina and sparing of the underlying choriocapillaris).²

Because the signs of the trauma are usually in the anterior portion of the eye, the posterior choroid is the best place to look for the granulomatous inflammation. Typically the neural retina is not involved, except near the ora serrata. Localized neural retinal detachments may be seen, especially in areas where Dalen–Fuchs nodules coalesce.

C. Other findings:

1. Tissue damage caused by the trauma

2. Extension of the granulomatous inflammation into the scleral canals and optic disc

Because uveal tissue is normally found in the scleral canals and in the vicinity of the optic disc, evisceration, which does not reach these areas, does not protect against sympathetic uveitis. If surgery is being done to prevent sympathetic uveitis, the procedure must be an enucleation, not an evisceration.

Phacoanaphylactic (Phacoimmune, Phacoantigenic, or Phacogenic) Endophthalmitis

I. Phacoanaphylactic endophthalmitis (PE) (Fig. 4.3) is a rare, autoimmune, unilateral (sometimes bilateral if the lens capsule is ruptured in each eye), zonal, granulomatous inflammation centered around lens material. It depends on a ruptured lens capsule for its development.

Fig. 4.3 Phacoanaphylactic endophthalmitis. A, The patient had an iridencleisis in 1971. The eye was injured by blunt trauma in an automobile accident in May 1973. In September 1973, signs of an anterior uveitis developed. Note the small mutton-fat keratic precipitates just to the right of the corneal slit-lamp section in the lower third of the picture. The eye was enucleated in May 1974. B, The enucleated globe shows iris in the subconjunctival tissue. The lens remnant, mainly nucleus, shows a zonal type of granulomatous reaction, consisting of surrounding epithelioid cells and giant cells, in turn surrounded by lymphocytes and plasma cells, in turn surrounded by granulation tissue. The lens capsule is ruptured posteriorly. C, Under increased magnification, the typical zonal pattern is seen around the remnant of the lens nucleus (periodic acid–Schiff stain).

II. The disease occurs under special conditions that involve an abrogation of tolerance to lens protein.

A. PE may result from the breakdown or reversal of central tolerance at the T-cell level. Small amounts of circulating lens protein normally maintain T-cell tolerance, but it may be altered as a result of trauma, possibly through the adjuvant effects of wound contamination or bacterial products or both.

B. After the abrogation of tolerance to lens protein, antilens antibodies are produced. The antibodies reach the lens remnants in the eye, and an antibody–antigen reaction takes place (PE).

Presumably, the lens protein that leaks through an intact capsule (e.g., in a mature or hypermature lens) is denatured (unlike the nondenatured lens protein that escapes through a ruptured lens capsule). Thus, it is incapable of acting as an antigen and eliciting an antibody response. The denatured lens protein, however, may incite a mild foreign-body macrophagic response. The macrophages, swollen with engulfed denatured lens material, may block the anterior chamber drainage angle and cause an acute secondary open-angle glaucoma called phacolytic glaucoma (see Chapter 10).

III. Histologically, in addition to the findings at the site of injury, a zonal granulomatous inflammation is found.

A. Activated neutrophils surround and seem to dissolve or eat away lens material, releasing proteolytic enzymes, arachidonic acid metabolites, and oxygen-derived free radicals.

B. Epithelioid cells and occasional (sometimes in abundance) multinucleated inflammatory giant cells are seen beyond the neutrophils.

C. Lymphocytes, plasma cells, fibroblasts, and blood vessels (granulation tissue) surround the epithelioid cells.

D. Usually the iris is encased in, and inseparable from, the inflammatory reaction.

E. The uveal tract usually shows a reactive, chronic nongranulomatous inflammatory reaction. Sometimes, however, the same trauma that ruptures the lens and sets off the PE initiates a sympathetic uveitis and results in a diffuse, chronic, granulomatous inflammation.

Foreign-Body Granulomas

I. Foreign-body granulomas may develop around exogenous foreign bodies that are usually introduced into the eye at the time of a penetrating ocular wound, or they may develop around endogenous products such as cholesterol or blood in the vitreous.

An unusual cause of inflammatory granuloma of the conjunctiva is the synthetic fiber found in teddy bears, called a “teddy-bear” granuloma.

II. Rarely, blood in the vitreous incites a marked foreign-body inflammatory response. When this occurs, the intravitreal hemorrhage almost is invariably traumatic in origin rather than spontaneous.

III. Histologically, a zonal type of granulomatous inflammatory reaction surrounds the foreign body.

Nontraumatic Infections

Viral

I. Cytomegalic inclusion disease (salivary gland disease; Fig. 4.4)

A. Cytomegalic (CMV) inclusion disease is caused by systemic infection with the salivary gland virus, cytomegalovirus, an enveloped herpesvirus formed by an icosahedral capsid and a double-stranded DNA.

CMV is huge, containing more than 200 genes (compared with its modest relative, herpes simplex virus, which contains only 84 genes). It is estimated that CMV infects 80–85% of people by 40 years of age. In otherwise healthy, immunocompetent people, CMV infection usually runs a benign, asymptomatic course (rarely, a heterophile-negative mononucleosis syndrome occurs). After primary exposure, CMV may establish a latent infection and the virus genome may persist in cells undetectable by conventional culture assays.

1. Congenital: Characterized by retinochoroiditis, prematurity, jaundice, thrombocytopenia, anemia, hepatosplenomegaly, neurologic involvement, and intracranial calcification

CMV disease is the most common viral infection of the neonate, with an incidence of 5–20 per 1000 live births. Most of the infants are asymptomatic at birth. The differential diagnosis consists of the TORCH syndrome (see Chapter 3).

2. Acquired: Mainly found in patients whose immune mechanisms have been modified [e.g., acquired immunodeficiency syndrome (AIDS), acute leukemia, malignant lymphomas, chemotherapy, and immunosuppressive therapy for renal transplantation]

Patients who have AIDS and have a low CD4⁺ and CD8⁺ T-lymphocyte cell count are at a high risk for the development of CMV retinitis. In the antiviral therapy era, non-CMV ocular opportunistic infections are rare.

B. Clinically, a central retinochoroiditis, as seen in the congenital form, is similar to that seen in toxoplasmosis.

1. The acquired form starts with scattered, yellow-white retinal dots or granular patches that may become confluent and are associated with sheathing of adjacent vessels and retinal hemorrhages (characteristic hemorrhagic exudation with “pizza-pie” or “cottage cheese with catsup” appearance).

2. Neural retinal detachments may develop in 15% of affected eyes.

3. Other ocular findings include iridocyclitis, punctate keratitis, and optic neuritis.

A periphlebitis that mimics acute frosted retinitis may occur. Other conditions that may mimic CMV retinitis include other herpesviruses, measles, syphilis, fungal retinitis (Cryptococcus neoformans and Candida albicans), toxoplasmosis, and acute retinal necrosis.

4. Immune recovery uveitis, associated with potent antiviral therapies, refers to a condition in which heightened intraocular inflammation occurs in some patients who have pre-existing CMV retinitis.

C. Histologically, a primary coagulative necrotizing retinitis and a secondary diffuse granulomatous choroiditis are seen.

1. The infected neural retinal cells show large eosinophilic intranuclear inclusions and small, multiple, basophilic intracytoplasmic inclusions.

2. In areas of healed retinitis, clinically seen focal yellow-white plaques contain calcium when examined histologically.

The cytoplasmic inclusions consist of numerous virions closely associated with dense masses of matter (periodic acid–Schiff-positive on light microscopy) that are highly characteristic of CMV. An additional highly characteristic feature is the presence of the virions in a mass of viral subunit material that forms a lacy, centrally located pattern in the nucleus. The nucleolus is marginated and free of virions. Clumping of peripheral chromatin is lacking.

3. The location and character of the retinal vascular changes in AIDS indicate an ischemic pathogenesis, most profound in CMV retinitis.

Fig. 4.4 Cytomegalic inclusion disease. A, The fundus picture shows the characteristic sector hemorrhagic exudation (“pizza-pie” appearance) along the retinal vessels. B, Histologic section shows the relatively normal neural retina sharply demarcated on each side from the central area of coagulative retinal necrosis, secondary to the infection. The choroid shows a secondary mild and diffuse granulomatous inflammation. C, Increased magnification shows typical eosinophilic intranuclear inclusion bodies (ii) and small, round basophilic and cytoplasmic inclusion bodies (ci). (A, Courtesy of Dr. SH Sinclair; B and C, presented by Dr. Daniel Toussaint at the meeting of the Verhoeff Society, 1976.)

II. Varicella/herpes zoster virus (VZV; Figs 4.5 and 4.6)

A. VZV causes varicella (chickenpox) and herpes zoster (shingles).

1. The virus, a member of the herpesvirus family, consists of a lipid envelope surrounding an icosahedral nucleocapsid with a central, double-stranded DNA core; only the enveloped virions are infectious.

2. Congenital infection is rare (differential diagnosis consists of the TORCH syndrome; see Chapter 3).

3. In immunocompetent individuals, VZV is a major cause of the acute retinal necrosis syndrome (see Chapter 11).

Following herpes zoster ophthalmicus, patients have a nine times greater risk of developing cancer than patients without herpes zoster ophthalmicus. Also, the risk of anterior uveitis increases the year following VZV.

B. Ocular complications occur in approximately 50% of cases of herpes zoster ophthalmicus:

1. Cornea: dendritic ulcer (rare), ulceration, perforation, peripheral erosions, bullous keratopathy, epidermidalization (keratinization), band keratopathy, pannus formation, stromal vascularization, hypertrophy of corneal nerves, ring abscess, granulomatous reaction to Descemet’s membrane, and endothelial degeneration

2. Anterior segment: iridocyclitis followed by peripheral anterior synechiae, exudate, and hyphema

3. Iris: patchy necrosis and postnecrotic atrophy (mimics iris after attack of acute angle-closure glaucoma), chronic nongranulomatous inflammation, anterior-surface fibrovascular membrane, patchy necrosis of anterior portion of ciliary body, especially of circular and radial portions of ciliary muscle, and cataract and posterior synechiae

4. Posterior segment: chronic nongranulomatous choroiditis (commonly, granulomatous inflammation), retinal perivasculitis and vasculitis, mild mononuclear vitreal inflammatory infiltrate, and acute or chronic episcleritis and scleritis; optic nerve: perivasculitis and chronic leptomeningitis; and long posterior ciliary nerves and vessels: striking perineural and, less commonly, intraneural nongranulomatous and occasionally granulomatous inflammation and perivasculitis and vasculitis

C. Histologically, the most characteristic findings are lymphocytic (chronic nongranulomatous) infiltrations involving the posterior ciliary nerves and vessels, often in a segmental distribution, and a diffuse or patchy necrosis involving the iris and pars plicata of the ciliary body. Granulomatous inflammatory lesions also may be seen. Inclusion bodies have not been demonstrated in the chronic inflammatory lesions.

Fig. 4.5 Herpes zoster. A, Ophthalmic branch of trigeminal nerve involved, including tip of nose; the patient had iritis. B, Evisceration specimen from another patient who had herpes zoster ophthalmicus shows corneal thickening, scarring, and inflammation. C, Increased magnification shows granulomatous inflammation, with epithelioid cells and inflammatory giant cells, mainly centered around Descemet’s membrane (granulomatous reaction to Descemet’s membrane).

Fig. 4.6 Herpes zoster. A, Patient with herpes zoster ophthalmicus developed chronic herpes keratitis and then corneal perforation; the eye was enucleated. B, Nongranulomatous inflammatory infiltrates centered around ciliary nerves in posterior episclera (no granulomatous inflammation present), shown with increased magnification in C.

Bacterial

I. Tuberculosis (Mycobacterium tuberculosis; Figs 4.7 and 4.8)

A. Tuberculosis has re-emerged as a serious public health problem, mainly because of the human immunodeficiency virus (HIV) epidemic and newly developed resistance to standard antibiotic therapy.

It is estimated that approximately one-third of the world’s population is infected by Mycobacterium tuberculosis. It may present in children initially as a preceptal cellulitis unresponsive to systemic antibiotic therapy.

B. Tubercle bacilli reach the eye through the bloodstream, after lung infection.

Tubercle bacilli survive within macrophages because they secrete eukaryocyte-like serine/threonine protein kinase G within macrophage phagosomes, inhibiting phagosome–lysosome fusion and mediating intracellular survival of mycobacterium. Rarely, intraocular tuberculosis can occur without obvious systemic infection.

1. The most common form of ocular involvement is a cyclitis that rapidly becomes an iridocyclitis and may also spread posteriorly to cause a choroiditis.

2. Clinically, mutton-fat keratic precipitates are seen on the posterior surface of the cornea and deep infiltrates in the choroid, often in the posterior pole.

Retinal tuberculosis usually spreads from an underlying choroiditis. The involvement may become massive to form a large tuberculoma involving all the coats of the eye. Tuberculoprotein hypersensitivity may play a role in the pathogenesis of phlyctenules and Eales’ disease. Tuberculous choroiditis may simulate serpiginous choroiditis (called tuberculous serpiginous-like choroiditis).

C. Miliary tuberculosis usually causes a multifocal, discrete (sarcoidal, tuberculoidal) granulomatous choroiditis.

D. Histologically, the classic pattern of caseation necrosis consists of a zonal type of granulomatous reaction around the area of coagulative necrosis.

1. Smooth, acid-fast bacilli can be demonstrated by acid-fast (Ziehl–Neelsen) or fluorescent acid-fast stains.

2. The polymerase chain reaction, prepared from formaldehyde-fixed and paraffin-embedded tissue, can be helpful in making the diagnosis.

Fig. 4.7 Tuberculosis. A, Archeology college student presented with a granulomatous posterior choroiditis. Active pulmonary tuberculosis was documented; antituberculous therapy was instituted. Appearance of lesion 2 months (B) and 16 months (C) later. At time of last photograph (C), the tuberculosis was considered cured.

Fig. 4.8 Tuberculosis. A, Tuberculous zonal granuloma involves retina and choroid. Caseation necrosis is present. B, In the middle of the field, typical acid-fast organisms are shown by the Ziehl–Neelsen method. (Courtesy of Dr. AH Friedman.)

II. Leprosy (Hansen’s disease; M. leprae; Fig. 4.9)

A. In lepromatous leprosy, the lepromin test (analogous to the tuberculin test) is negative, suggesting little or no immunity. The prognosis is poor.

Genes belonging to the leukocyte immunoglobulin-like receptor (LIR) family are significantly upregulated in lesions of lepromatous patients suffering from the disseminated form of the infection.

1. Lepromas of the skin result in leonine facies and neurologic changes. The eyeballs are involved, usually in their anterior portions.

2. Histologically, a diffuse type of granulomatous inflammatory reaction, known as a leproma, is present.

a. Lepromas, which involve mainly cornea, anterior sclera, and iris, show large, pale-staining histiocytes that are called lepra cells when their cytoplasm is amorphous and Virchow’s cells when vacuolated.

b. The lepra cells and Virchow’s cells teem with beaded bacilli (no immunity).

The bacteria may grow better in the cooler, anterior portion of the eye rather than in the warmer, posterior portion, just as they do in the cooler skin instead of in the warmer, deeper structures of the body.

B. In tuberculoid leprosy, the lepromin test is positive, suggesting immunity. The prognosis is good.

1. A neural involvement, particularly the ulnar nerve (leads to claw hand), predominates with hypopigmented (vitiliginous), hypoesthetic lesions, and thickened nerves.

2. The ocular adnexa and orbital structures are involved, especially the ciliary nerves, but not the eyeballs.

3. Histologically, a discrete (sarcoidal, tuberculoidal) type of granulomatous inflammatory reaction is seen, mainly centered around nerves.

a. The individual nodules tend to be much more variably sized than those in sarcoidosis or miliary tuberculosis.

b. Organisms are extremely difficult to find (good immunity) and are usually located in an area of nerve degeneration.

Fig. 4.9 Lepromatous leprosy. A, Leonine facies present in patient who had lepromatous leprosy. Note involvement of left eye, shown with closer view in B. C, Many “clear” cells are seen with hematoxylin and eosin-stained section. D, Same cells are teeming with acid-fast leprous organisms (red) as seen with the Ziehl–Neelsen method. (A and B, Courtesy of Dr. B Blaise; C and D, courtesy of Dr. P Henkind.)

III. Syphilis (Treponema pallidum; Fig. 4.10)

A. Both the congenital and acquired forms of syphilis may produce a nongranulomatous interstitial keratitis (see Chapter 8) or anterior or posterior uveitis. Syphilis may occur in immunologically deficient patients (e.g., those with AIDS).

The two most commonly used nontreponemal tests (which detect antibody to cardiolipin–lecithin–cholesterol antigen) are the Venereal Disease Research Laboratory (VDRL) and the rapid plasma reagin. The treponemal tests (which detect antibody against treponemal antigens) include the fluorescent treponemal antibody absorption test (FTA-ABS), hemagglutination treponemal test for syphilis, T. pallidum hemagglutination assay, and the microhemagglutination test. Routine screening with VDRL and FTA-ABS may be considered in patients who have unexplained uveitis or other ocular inflammation.

B. Syphilis, a venereal disease, is divided into three chronologically overlapping stages.
The nonvenereal treponematoses caused by subspecies T. p. pertenue (yaws) and T. p. endemicum (bejel) are morphologically indistinguishable from T. pallidum and display only subtle immunologic differences.

1. Primary stage: Characterized by an ulcerative lesion, chancre, occurring at the site where T. pallidum penetrates the skin or mucous membrane. Primary lesions heal spontaneously in 2–8 weeks and rarely cause systemic symptoms.

2. Secondary stage: The period when the systemic treponemal concentration is greatest, usually 2–12 weeks after contact.

a. This stage may be manifest by fever, malaise, lymphadenopathy, and mucocutaneous lesions.

b. The secondary stage subsides in weeks to months but may recur within 1–4 years.

3. Tertiary stage: The late sequelae such as cardiovascular effects and neurosyphilis. Focal granulomatous vascular lesions (gummas) can affect any organ.

C. The common form of posterior uveitis is a smoldering, indolent, chronic, nongranulomatous inflammation.

1. Disseminated, large, atrophic scars surrounded by hyperplastic retinal pigment epithelium (part of the differential diagnosis of “salt-and-pepper” fundus) characterize the lesions.

2. A more virulent type of uveitis may occur with a granulomatous inflammation.

D. Histologic findings

1. The chronic nongranulomatous disseminated form of posterior choroiditis:

a. In the atrophic scar, the outer neural retinal layers, the retinal pigment epithelium, and the inner choroidal layers disappear. Dehiscences in Bruch’s membrane may be present through which neural retinal elements may “invade” the choroid.

b. Bruch’s membrane may be folded into the atrophic, sclerosed choroid. Scattered lymphocytes and plasma cells may be present.

c. The Treponema spirochete is a helical bacterium 5–15 µm in length and less than 0.18 µm in width, and it can be demonstrated in the ocular tissue with special stains, often in areas devoid of inflammatory cells.

Treponema pallidum belongs to the same family (Spirochaetaceae) as Borrelia (see later) and Leptospira.

2. The granulomatous form of posterior chorioretinitis:

a. The inflammatory process usually involves the choroid and the overlying neural retina and is quite vascular. Epithelioid cells, lymphocytes, and plasma cells are seen.

b. Spirochetes can be demonstrated in the inflammatory tissue.

3. The preceding two types of reactions may also involve the anterior uvea.

Spirochetes may be obtained by aspiration of aqueous from the anterior chamber and identified by dark-field microscopy.

Fig. 4.10 Syphilis. A, Small, round translucent nodules are seen in the conjunctiva of the inferior fornix. B, Biopsy of nodules shows numerous granulomas under the conjunctival epithelium (ce, surface conjunctival epithelium; gr, granulomatous reaction in substantia propia). C, Increased magnification reveals epithelioid cells in the inflammatory nodules. D, A special stain, Dieteria, demonstrates spirochetes (s) in the inflammatory infiltrate. (Case reported in Spektor FE, Eagle RC, Nichols CW: Granulomatous conjunctivitis secondary to Treponema pallidum. Ophthalmology 88:863. © PubMed Central 1981.)

IV. Lyme disease (Borrelia burgdorferi; Fig. 4.11)

A. Lyme disease is a worldwide, tickborne, multisystem disorder, heralded by a red rash and erythema migrans, which forms at the site of the tick bite, usually within 4–20 days. The rash enlarges with central clearing (forming a ring), can last several weeks, and may return and become chronic (erythema chronicum migrans).

B. The tick, an Ixodes species, transmits the infectious agent, B. burgdorferi, through its bite. The enzyme-linked immunosorbent assay (ELISA) and the indirect immunofluorescence antibody are the most commonly used diagnostic tests.

C. Like syphilis, Lyme disease is divided into three chronologically overlapping stages. Not all patients exhibit each stage, and the signs and symptoms are variable within each stage.

1. Stage 1 is characterized by the local erythema migrans, which may be accompanied by flulike symptoms, including headache, fever, malaise, and lymphadenopathy. Ocular findings include follicular conjunctivitis and photophobia.

2. Stage 2 occurs within days, weeks, or even months and reflects systemic dissemination of the spirochete.

a. Multiple skin lesions may occur (e.g., the purple nodule, lymphocytoma, especially on the earlobe or breast).

b. Other findings include cardiac problems, arthritis (rare in stage 2), and the neurologic triad of meningitis, cranial neuritis, and painful radiculitis.

c. Ocular findings include blepharitis, blepharospasm, iridocyclitis, uveitis, neuroretinitis, vitritis, pars planitis, macular edema, anterior ischemic optic neuropathy (ANION), optic neuritis, optic neuropathy, temporal arteritis, pseudotumor cerebri, optic disc edema, optic disc pallor, cranial ocular nerve palsies, Horner’s syndrome, and Argyll–Robertson pupil.

3. Stage 3 can follow a disease-free period and may last years.

a. “Lyme arthritis” is the hallmark of stage 3, appearing in more than 50% of untreated cases.

b. Other findings include acrodermatitis chronica atrophicans and late neurologic sequelae, especially an encephalopathy.

c. Ocular findings include stromal keratitis, episcleritis, orbital myositis, and cortical blindness.

D. The pathologic mechanisms include direct invasion of tissues by the spirochete, vasculitis and small-vessel obliteration, perivascular plasma cell infiltration, and immunologic reactions.

Fig. 4.11 Lyme disease. Lyme disease can cause a choroiditis (A) or an optic neuritis (B and C). Another patient had a chronic bilateral uveitis with a unilateral exudative retinal detachment and inflammatory pupillary membrane. Surgical iridectomy and membrane excision were performed. Spirochetes (Borrelia burgdorferi) were demonstrated by silver stain (D) and cultured in MKP medium (in the 16th subculture) from the excised tissue. (A–C, Courtesy of Prof. GOH Naumann; D, courtesy of Prof. HE Völcker and reported by Preac-Mursic V, Pfister HW, Spiegel H et al.: First isolation of Borrelia burgdorferi from an iris biopsy. J Clin Neuroophthalmol 13:155. © North American Neuro-Ophthalmology Society 1993.)

V. Streptothrix (Actinomyces; Fig. 4.12)

A. The organism responsible for streptothrix infection of the lacrimal sac (see Chapter 6) and for a chronic form of conjunctivitis belongs to the class Schizomycetes, which contains the genera Actinomyces and Nocardia. The organism superficially resembles a fungus, but it is a bacterium, best classified as an anaerobic and facultative capnophilic bacterium of the genus Actinomyces.

B. Histologically, the organisms (weakly gram-positive and acid-fast) are seen in colonies as delicate, branching, intertwined filaments surrounded by necrotic tissue with little or no inflammatory component (e.g., the lacrimal cast from the nasolacrimal system).

The colonies can be seen macroscopically as gray or yellow “sulfur” granules. Inflammatory giant cells are seen occasionally.

Fig. 4.12 Streptothrix (Actinomyces). A, Clinical appearance of acute canaliculitis. B, Smear of lacrimal cast stained with peridic acid–Schiff shows large colonies of organisms. (A, Courtesy of Dr. HG Scheie.)

VI. Cat-scratch disease [CSD: Bartonella (previously called Rochalimaea) henselae, cat-scratch bacillus]

A. CSD is a subacute regional lymphadenitis following a scratch by a kitten or cat (or perhaps a bite from the cat flea, Ctenocephalides felis), caused by the cat-scratch bacillus, B. henselae, a slow-growing, fastidious, gram-negative, pleomorphic bacillus, which is a member of the α₂ subgroup of the class Probacteria, order Rickettsiales, family Rickettsiaceae.

1. Systemic manifestations in severe cases include splenohepatomegaly, splenic abscesses, mediastinal masses, encephalopathy, and osteolytic lesions.

2. Ocular findings include Parinaud’s oculoglandular fever (see Chapter 7), neuroretinitis, branch retinal artery or vein occlusion, multifocal retinitis (retinal white-dot syndrome), focal choroiditis, intraretinal white spots, macular hole, optic disc edema associated with peripapillary serous retinal detachment, optic nerve head inflammation, and orbital infiltrates.